Recombinant protein folding and misfolding in Escherichia coli
Article Abstract:
The mechanisms used by Escherichia coli to support protein folding, manage stress-induced protein misfolding are also used to promote high-level production of heterologous proteins in a correct and bioactive conformation in the bacterial cytoplasm and periplasm. The principles that govern protein folding and misfolding in E. coli, the availability of sophisticated tools for chromosome engineering and rapid progress in PCR are described.
Publication Name: Nature Biotechnology
Subject: Business
ISSN: 1087-0156
Year: 2004
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Construction of lycopene-overproducing E. coli strains by combining systematic and combinatorial gene knockout targets
Article Abstract:
An important problem in industrial strain construction and a central tenet of metabolic engineering is the identification of genes that affect the product accumulation phenotype of recombinant strains. A study identifies gene knockout targets that increase lycopene biosynthesis in strains of Escherichia coli by using systematic (model-based) and combinatorial (transposon-based) methods.
Publication Name: Nature Biotechnology
Subject: Business
ISSN: 1087-0156
Year: 2005
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