Adsorption and activity of a domoic acid binding antibody fragment on mesoporous silicates

Article Abstract:

The adsorption of an anti-domoic acid single-chain Fv (scFv) antibody fragment onto a range of mesoporous silicate supports was investigated. The scFv antibody fragment was immobilized onto mesoporous silicates of varying surface areas and pore diameters, but the isoelectric points of the antibody fragment and adsorbent material were found to be the most important determinant of protein adsorption.

All Other Miscellaneous Chemical Product and Preparation Manufacturing, Adsorbents, Chemical preparations, not elsewhere classified, Analysis, Protein research, Adsorption

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Mechanism of dihydroneopterin aldolase: A molecular dynamics study of the apo enzyme and its product complex

Article Abstract:

A series of molecular dynamic simulations is performed for the study of an apo enzyme, dihydroneopterin aldolase (DHNA) in its free as well as complexed form. The analysis shows that the pathway chosen by the hydroprotein provides a barrier consistent with the rate of product release and leads to a minimal disturbance of the system.

Proteins, Fructosediphosphate aldolase, Aldolases

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Methodology for the immobilization of enzymes onto mesoporous materials

Article Abstract:

A methodical order was developed whereby the influences of surface area, pore size, extent of order, particle size, surface potentials, isoelectric points, pH, and ionic strength on immobilization were explored. A systematic methodology for the adsorption of proteins onto mesoporous silicates is described.

Cytochrome c

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