Adsorption and diffusion of single molecules at chromatographic interfaces

Article Abstract:

The contributions of single molecule spectroscopy to the understanding of an important problem in chromatography are detailed. These single molecule experiments provide important new information about the so called 'active silanols' that cause tailing to persist at low pH, revealing that these sites are neutral and that surface topography plays a role.

Chemistry, Physical and theoretical, Physical chemistry, Chromatography, Spectra (Spectroscopy)

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NMR and computational studies of chiral discrimination by amylose tris(3,5-dimethylphenylcarbamate)

Article Abstract:

Proton NMR and simulations are combined to examine the origin of chiral selectivity by a polysaccharide used in a commercial chromatographic stationary phase: amylase tris(3,5-dimethylphenylcarbamate). 2D NOESY spectra showed which protons of each enantiomer and the polysaccharide were in proximity, and revealed folding of the L-enantiomer.

All Other Basic Organic Chemical Manufacturing, Industrial organic chemicals, not elsewhere classified, Polysaccharides, Nuclear magnetic resonance spectroscopy, Chirality, Protons, High performance liquid chromatography, Structure

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Single-molecule study of an adsorbed oligonucleotide undergoing both lateral diffusion and strong adsorption

Article Abstract:

Single-molecule fluorescence spectroscopy was used to probe the lateral transport of an adsorbed oligonucleotide, the SP6 promoter primer, which is a 24-mer labeled at the 5'end with tetramethylrhodamine. Oligonucleotides captured by this surface would undergo fast diffusion that would facilitate hybridization.

Adsorption, Fluorescence spectroscopy, DNA microarrays

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