(CdSe)ZnS quantum dots and organophosphorus hydrolase bioconjugate as biosensors for detection of paraoxon
Article Abstract:
A novel biosensor for the detection of the paraoxon, based on (CdSe)ZnS core-shell quantum dots (QDs) and an organophosphorus hydrolase (OPH) biconjugate, was reported. The OPH was coupled to (CdSe)ZnS core-shell QDs through electrostatic interaction between negatively charged QDs surfaces and the positively charged protein side chain and ending groups.
Publication Name: Journal of Physical Chemistry B
Subject: Chemicals, plastics and rubber industries
ISSN: 1520-6106
Year: 2005
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Layer-by-Layer films of chitosan, organophosphorus hydrolase and thioglycolic acid-capped CdSe quantum dots for the detection of paraoxon
Article Abstract:
The versatility of the layer-by-layer (LbL) system is explored where the multilayers of chitosan (CS), thioglycolic acid (TGA)-capped CdSe QDs, and OPH polyelectrolytes are incorporated into the LbL architecture. The fabricated LbL thin film showed an attractive alternative to using polyelectrolytes with optical properties for biosensing assembly use.
Publication Name: Journal of Physical Chemistry B
Subject: Chemicals, plastics and rubber industries
ISSN: 1520-6106
Year: 2003
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Secondary structure of organophosphorus hydrolase in solution and in Langmuir-Blodgett film studied by circular dichroism spectroscopy
Article Abstract:
The secondary structure of organophosphorus hydrolase (OPH) has been studied with circular dichroism spectroscopy. The effect of pH on the secondary structure of OPH solution has been examined over the pH range from 3.56 to 9.60.
Publication Name: Journal of Physical Chemistry B
Subject: Chemicals, plastics and rubber industries
ISSN: 1520-6106
Year: 2004
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