FRET fluctuation spectroscopy: exploring the conformational dynamics of a DNA hairpin loop
Article Abstract:
The large charges in proximity ratio from hairpin to duplex support the conclusion that fluctuations in florescence, and hence the observed complex dynamics, are dominated by conformational changes of the DNA, not other possible fluctuations phenomena. The simple experiment demonstrates the potential of fluorescence resonance energy transfer fluctuation spectroscopy to understand the conformational dynamics of nucleic acids.
Publication Name: Journal of Physical Chemistry B
Subject: Chemicals, plastics and rubber industries
ISSN: 1520-6106
Year: 2000
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Probing the complete folding trajectory of a DNA hairpin using dual beam fluorescence fluctuation spectroscopy
Article Abstract:
A dual probe beam fluorescence fluctuation spectroscopy was used to study the conformational fluctuations of dye-quencher labeled DNA hairpin molecules in aqueous solution. The intermediate states of the DNA hairpins are found to be influenced by a collapsed ensemble of folded hairpins containing various partially folded and misfolded conformations.
Publication Name: Journal of Physical Chemistry B
Subject: Chemicals, plastics and rubber industries
ISSN: 1520-6106
Year: 2008
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Ratiometric analysis of single-molecule fluorescence resonance energy transfer using logical combinations of threshold criteria: a study of 12-mer DNA
Article Abstract:
Research is presented describing the study of 12-mer DNA to determine fluorescence energy transfer rates of single molecules.
Publication Name: Journal of Physical Chemistry B
Subject: Chemicals, plastics and rubber industries
ISSN: 1520-6106
Year: 2000
User Contributions:
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