On the steady-state assumption and its application to the rotating disk voltammetry of adsorbed enzymes
Article Abstract:
Rotating disk voltammetry is routinely used to study the electrochemically driven enzyme catalysis because of the assumption that the method produces a steady-state system. It is concluded that under typical experimental conditions, steady-state enzyme catalysis is unlikely to occur within electrode-immobilized enzyme layers and that typically sigmoidal rotating disk voltammograms merely reflect a mass transfer steady as opposed to a true steady state of enzyme intermediates at each potential.
Publication Name: Journal of Physical Chemistry B
Subject: Chemicals, plastics and rubber industries
ISSN: 1520-6106
Year: 2005
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Rates of catalyzed processes in enzymes and other cooperative media
Article Abstract:
The traditional transition stat theory explains catalysis in terms of reduced barriers and result state reaction barriers as intrinsic properties. The considerable rate enhancements exhibited by enzyme-catalyzed reactions with respect to the corresponding processes in solution are interpreted as a consequence of the enhanced coupling of active modes to the reaction coordinate.
Publication Name: Journal of Physical Chemistry B
Subject: Chemicals, plastics and rubber industries
ISSN: 1520-6106
Year: 2003
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Single-molecule Michaelis-Menten equations
Article Abstract:
Models based on the Michaelis-Menten mechanism are presented to account for the experimental observations. The single-molecule Michaelis-Menten equation in the absence of dynamic disorder starting from the differential equations that define both the ensemble-averaged and single-molecule Michaelis-Menten kinetics is discussed.
Publication Name: Journal of Physical Chemistry B
Subject: Chemicals, plastics and rubber industries
ISSN: 1520-6106
Year: 2005
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