Two-dimensional attenuated total reflection/infrared correlation spectroscopy of adsorption-induced and concentration-dependent spectral variations of beta-lactoglobulin in aqueous solutions
Article Abstract:
The potential of generalized two-dimensional (2D) attenuated total reflection/infrared (ATR/IR) spectroscopy in studies of spectral variations in the amide I region of aqueous solutions of protein are demonstrated. The 2D map reveals that the intensity changes observed for the series of aqueous solutions of beta-lactoglobulin with different concentrations are predominantly composed of concentration-induced secondary structure changes.
Publication Name: Journal of Physical Chemistry B
Subject: Chemicals, plastics and rubber industries
ISSN: 1520-6106
Year: 2000
User Contributions:
Comment about this article or add new information about this topic:
Two-dimensional/attenuated total reflection infrared correlation spectroscopy studies on secondary structural changes in human serum albumin in aqueous solutions: pH-dependent structural changes in the secondary structures and in the hydrogen bondings of side chains
Article Abstract:
The acid unfolding process of human serum albumin (HSA) by use of a generalized two-dimensional attenuated total reflection infrared correlation spectroscopy. The Two-dimensional correlation spectra have yielded information of secondary structural changes and changes in the COO(super -) and COOH groups and other side chains.
Publication Name: Journal of Physical Chemistry B
Subject: Chemicals, plastics and rubber industries
ISSN: 1520-6106
Year: 2001
User Contributions:
Comment about this article or add new information about this topic:
Two-dimensional near-infrared spectroscopy study of human serum albumin in aqueous solutions: using overtones and combination modes to monitor temperature-dependent changes in the secondary structure
Article Abstract:
FT-NIR spectra were measured for human serum albumim (HSA) in aqueous solutions with concentrations of 1.0, 2.0, 3.0, 4.0 and 5.0 wt % over a temperature range of 45-80 degree celsius. Results reveal that the overtone and combination modes are very useful in monitoring subtle changes in protein dynamics.
Publication Name: Journal of Physical Chemistry B
Subject: Chemicals, plastics and rubber industries
ISSN: 1520-6106
Year: 2000
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Two-dimensional near-infrared correlation spectroscopy studies of compatible polymer blends: composition-dependent spectral variations of blends of atactic polystyrene and poly(2,6-dimethyl-1,4-phenylene ether)
- Abstracts: In situ attenuated total reflection infrared spectroscopy: a sensitive tool for the investigation of reduction- oxidation processes on heterogeneous Pd metal catalysts
- Abstracts: Epitaxial assembly in aged colloids. A combined temperature-calorimetric study of ion adsorption at the hematite-electrolyte interface: II. Models of a heterogeneous oxide surface
- Abstracts: Insights into hydrogen atom adsorption on and the electrochemical properties of nitrogen-substituted carbon materials
- Abstracts: Surface characterization using metastable impact electron spectroscopy of adsorbed xenon. Water and methanol adsorption on MgO(100) studied by electron spectroscopies and thermal programmed desorption