Two types of protein hydration measured by dielectric dispersion in the gigahertz region, and effects of anesthetics

Article Abstract:

The number of the strongly bound water molecules (N(sub s)) and the weakly bound water molecules (N(sub w)) bound to bovine serum albumin (BSA) are estimated by the microwave impedance dispersion to understand the effect of a volatile anesthetic halothane on the protein hydration. The increase of conductance above 1.0 nM halothane indicates unfolding of the protein, exposing polar moieties to water, thus increasing the ion concentration.

Chemistry, Physical and theoretical, Physical chemistry

---

Directed assembly of binary monolayers with a high protein affinity: Infrared reflection absorption spectroscopy (IRRAS) and surface plasmon resonance (SPR)

Article Abstract:

Infrared reflection absorption spectroscopy (IRRAS) and surface plasmon resonance (SPR) techniques are used to investigate human serum albumin (HSA) binding to binary monolayers of zwitterionic dipalmitoylphosphatidylcholine (DPPC) and cationic diactadecyldimethylammonium bromide (DOMA). The two components in the monolayers deliver through lateral reorganization to form multiple interaction sites favorable for protein binding.

Usage, Monomolecular films, Infrared spectroscopy, Choline, Affinity labeling, Structure, Report

---

Effects of pyrophosphate ions of protein adsorption onto calcium hydroxyapatite

Article Abstract:

The effects of pyrophosphate ions on the adsorption of proteins onto calcium hydroxyapatite (Hap) are examined by using typical proteins of bovine serum albumin (BSA), myoglobin (MGB) and lysozyme (LSZ). The results of the zeta potential for all the protein systems have supported the modes of protein adsorption.

Analysis, Adsorption, Mechanical properties, Pyrophosphates

Similar abstracts:

• Abstracts: Molecular dynamics of carbohydrate aqueous solutions. dielectric relaxation as a function of glucose and fructose concentration
• Abstracts: Probing the contribution of electronic coupling to the directionality of electron transfer in photosynthetic reaction centers
• Abstracts: Vibrational circular dichroism, absolute configuration, and predominant conformations of volatile anesthetics: desflurane
• Abstracts: Enhanced intra-aggregate charge separation from binary exictons in mixed J-aggregates of cyanine dyes. Direct voltammetric observation of reversible and irreversible oxidations of two-dimensional J-aggregates of cyanine dye
• Abstracts: Effect of protein environment of pK(sub a) shifts in the active site of photoactive yellow protein. Study of the opsin shift of bacteriorhodopsin: Insight from QM/MM calculations with electronic polarization effects of the protein environment

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.