X-ray absorption microscopy of bacterial surface protein layers: x-ray damage
Article Abstract:
The electronic structure of individual sheets of the bacterial surface protein layer of Bacillus sphaericus NCTC 9602 is analyzed using a photoemission electron microscope (PEEM) operating in near-edge X-ray absorption fine structure spectroscopy mode. An irradiation-induced increase of the C-C bond density is observed at the cost of the densities of the C-O and C-N bonds that is related to a rearrangement of the contributing atoms of the S layer deposited onto a Si substrate.
Publication Name: Journal of Physical Chemistry B
Subject: Chemicals, plastics and rubber industries
ISSN: 1520-6106
Year: 2007
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Oriented assembly of purple membrane on solid support, mediated by molecular recognition
Article Abstract:
A triple layer of the biotin-lipid/streptavidin/biotinylated purple membrane was assembled by specific molecular recognition to test its properties. It is found that the photochemical activity of the purple membrane was hardly affected by biotin labeling. Electron Microscope (EM) visualized the assembly procedure, and Surface Plasmon Resonance (SPR) showed precise data of streptavidin and purple membrane adsorbed.
Publication Name: Journal of Physical Chemistry B
Subject: Chemicals, plastics and rubber industries
ISSN: 1520-6106
Year: 2003
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Structural changes of purple membrane and bacteriorhodopsin during its denaturation induced by high pH
Article Abstract:
The denaturation bacteriorhodopsin (bR) is found to occur during a very narrow pH range when the pH is increased above 12.0, as indicated by inactivation of the photochemical cycle observed by flash photolysis kinetic spectra. Atomic force microscopy is used to study the surface structural changes of purple membrane (PM) during the denaturation process induced by high pH.
Publication Name: Journal of Physical Chemistry B
Subject: Chemicals, plastics and rubber industries
ISSN: 1520-6106
Year: 2005
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- Abstracts: X-ray absorption spectromicroscopy study of UV-photoinduced surface modification and anisotropy in polyimide films
- Abstracts: Estimation of dielectric function of biotin-capped gold nanoparticles via signal enhancement on surface plasmon resonance
- Abstracts: Multiple proton-transfer reactions in DNA base pairs by coordination of Pt complex. Spatial and mechanical properties of dilute DNA monolayers on gold imaged by AFM