Proteoglycans in articular cartilage revealed with a quick freezing and deep etching method
Article Abstract:
A quick-freezing and deep etching (QF-DE) method of preparing joint cartilage samples appears to be effective for preserving and visualizing three-dimensional proteoglycan structures. Proteoglycans are sugar-protein compounds present in cartilage and connective tissues. Hip joint cartilage samples from rats were analyzed using QF-DE, staining, and conventional techniques. Lattice and strand-like details suggestive of proteoglycan, collagen, and aggrecans structures were visualized using the QF-DE method. A washing and fixing step in the QF-DE procedure was important to preserve the details of these structures.
Publication Name: Annals of the Rheumatic Diseases
Subject: Health
ISSN: 0003-4967
Year: 1996
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Effects of methotrexate on normal articular cartilage in vitro and in vivo
Article Abstract:
Methotrexate does not appear to have adverse effects on joint cartilage. Researchers exposed joint cartilage samples from cows to methotrexate and also gave methotrexate or a placebo to 48 rabbits. Methotrexate had little adverse effect on cartilage but did affect some metabolic functions. However, this occurred at doses much higher than those found in the joint fluid of patients with rheumatoid arthritis who take methotrexate.
Publication Name: Annals of the Rheumatic Diseases
Subject: Health
ISSN: 0003-4967
Year: 1998
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