Affinity maturation leads to differential expression of multiple copies of a kappa light-chain transgene
Article Abstract:
Hpermutation was studied in transgenic mice with rearranged heavy or light chains. Hybridomas from secondary responses expressing a transgene which codes for a light chain produced in response to the hapten 2-phenyloxazolone were chosen and the expression of five transgene copies in these hybridomas were studied. Results revealed that antibody light-chain transgene expression was affected by the hypermutation process. Cells downregulating transgene copies at several levels of gene expression were also found to be favored by antigenic selection when mutations capable of enhancing antibody affinity appear in one of the copies.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 1993
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Original and artificial antibodies
Article Abstract:
Hybridomas that immortalize B cells through cell fusion are preferable to PCR cloning of variable genes as a means of producing monoclonal antibodies that are effective binders. Comparison of the anti-2-phenyloxazolone antibodies yielded by hybridomas with single chain Fvs derived with messenger RNA from one group of spleen cells indicated that hybridomas were the more efficient method of making usable antibodies.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 1992
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Codon bias targets mutation
Article Abstract:
AGY codons in the complementarity determining regions and TCN codons in the frameworks of human immunoglobulin variable region gene segments are preferred as revealed by the analyses of immunoglobulin V genes in humans and Xenopus. Serine AGY codons showed more mutability than serine TCNs in protein coding sequences of humans and mice. Some exceptions to this biased codon selection are discussed.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 1995
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