An RNA-directed nuclease mediates post-transcriptional gene silencing in Drosophila cells
Article Abstract:
The post-transcriptional element of double-stranded RNA-dependent gene silencing seems to be carried out by a sequence-specific nuclease incorporating these small RNAs as guides that target specific messages based on sequence recognition. S2 cells were transfected with a double-stranded RNA corresponding to the first 540 nucleotides of Drosophila cyclin E, a gene that is vital for progression into S phase of the cell cycle, in order to establish whether RNA interference could be used to target endogenous genes. It is now vital to establish whether conservation of gene-silencing mechanisms exists at the transcriptional level.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 2000
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Role for a bidentate ribonuclease in the initiation step of RNA interference
Article Abstract:
The 'dicer' enzyme has been identified that can produce putative guide RNAs.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 2001
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A microRNA polycistron as a potential human oncogene
Article Abstract:
B-cell lymphoma samples and cell lines to normal tissues are compared and reveal that the levels of the primary or mature microRNAs derived from the mir 17-92 locus are often substantially increased in cancer. Enforced expression of the mir-17-92 cluster acted with c-myc expression to accelerate tumor development in a mouse B-cell lymphoma model.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 2005
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