Evolution in vitro of an RNA enzyme with altered metal dependence
Article Abstract:
Artificial phenotypes of the Tetrahymena group I ribozyme were produced using in vitro evolution techniques. This ribozyme is an RNA enzyme responsible for catalyzing a sequence-specific phosphodiester cleavage reaction on an external RNA oligonucleotide substrate when near a divalent metal cation cofactor. The new genetic variants of the ribozyme can use calcium as the divalent cation rather than magnesium or manganese as is normal. The artificial phylogenic change resembles the effects of natural selection.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 1993
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A ribozyme that lacks cytidine
Article Abstract:
It has been established that even three nucleic-acid subunits are sufficient to provide a large rise in the catalytic rate. This research used in vitro evolution to produce an RNA ligase ribozyme that lacks cytidine. Ribozymes lacking cytidine indicate that evolution can deal with an extremely limited set of chemical building blocks in producing macromolecules with a complex structure and function. It is clear that RNA folding and catalysis can be achieved in a three-nucleotide system.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 1999
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Leslie Orgel
Article Abstract:
Leslie Orgel, the chemist and molecular biologist, was known for his pioneering contribution to study of the origins of life.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 2007
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