Expression cloning and characterization of a renal electrogenic Na+/HCO3 cotransporter
Article Abstract:
It is possible to follow the expression of the renal 1:3 electrogenic Na+/HCO3 cotransporter from the salamander Ambystoma tigrinum, the most electrogenic transporter known, by monitoring the intracellular pH and membrance voltage in Xenopus oocytes. Researchers have been able to clone and characterize a cDNA encoding a cation-coupled HCO3 transporter. This protein can be seen to be electrogenic, Na+ and HCO3-dependent and blocked by the anion-transport inhibitor DIDS when expressed in Xenopus oocytes. The cloned transporter may play a useful role in the molecular identification of other cation-coupled HCO3 transporters.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 1997
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Cloning and characterization of a mammalian proton-coupled metal-ion transporter
Article Abstract:
Researchers looking at metal ions as vital cofactors in a range of biological processes have identified DCT1, a new metal-ion transporter in the rat. This has a very wide substrate range, and mediates active transport which is proton-coupled and relies on the cell membrane potential. It is upregulated by dietary iron deficiency, and belongs to the 'natural-resistance-associated macrophage protein' family. This work makes a valuable contribution to the understanding of how divalent cations cross cell membranes, especially those of epithelia.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 1997
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A family of mammalian Na+-dependent L-ascorbic acid transporters
Article Abstract:
Vitamin C (L-ascorbic acid) is required for certain enzymatic reactions. Two L-ascorbic acid transporters, SVCT1 and SVCT2 have been isolated from rate complementary DNA libraries to study the importance of L-ascorbic acid transport in supply and metabolism regulation of vitamin C. Both transporters mediate high affinity L-ascorbic acid transport driven by the Na+ electrochemical gradient.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 1999
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