Intersubunit rotation in active F-ATPase
Article Abstract:
Gamma subunit of the active peripheral portions of F-ATPase from spinach chloroplast rotates relative to the stationary (alpha-beta)(sub 3) subunit during the hydrolysis of ATP by the ATPase. Polarized absorption relaxation of the photobleached and immobilized enzyme shows that the rotation has an angular range of at least 200 degrees and a time constant of 100 ms. The time constant agrees with the rate of ATP hydrolysis. Hydrolysis is probably by a triple-site method and the gamma subunit functions as a crankshaft in the (alpha-beta)(sub 3) subunit.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 1996
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Connecting stalks in V-type ATPase
Article Abstract:
It has been possible to identify a more complete structure of the V-type ATPase stator moiety. It was established that the intact V-type ATPase has a central stalk and two stator connections. The stators are likely to be the bacterial V-type subunits I, E and F. This is the first time that two stators have been observed, possibly because the intact structure is vulnerable to damage on preparation. It is possible that the two stators are unique for V-type ATPases as, in the stalk region of F-type ATPases, only the b subunit exists in two copies.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 1999
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Light-driven production of ATP catalysed by F0F1-ATP synthase in an artificial photosynthetic membrane
Article Abstract:
Light usage by plants and photosynthetic bacteria involves the production of a transmembrane proton gradient which can drive biochemical synthesis, as well as transport and mechanical processes. A new study incorporates F0F1-ATP synthase from spinach leaf chloroplasts into a liposome bilayer containing parts of the proton-pumping photocycle. The system resembles the way that light is converted into ATP by photosynthetic bacteria.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 1998
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