Laboratory evolution of peroxide-mediated cytochrome P450 hydroxylation
Article Abstract:
The desire to create practical single-enzyme hydroxylation catalysts has prompted efforts to create cytochrome P450s that efficiently use hydrogen peroxide instead of dioxygen and NADH. This work has involved the directed evolution of the P450 from Pseudomonas putida to form mutants that hydroxylate naphthalene where no cofactors are present through the 'peroxide shunt' pathway. Activity is more than 20-fold higher than the native enzyme. Further evolution of P450s would make it possible to investigate the limits of P450-catalyzed aromatic hydroxylation through the peroxide shunt pathway.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 1999
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A synthetic multicellular system for programmed pattern formation
Article Abstract:
A synthetic multicellular system in which genetically engineered 'receiver' cells are programmed to form ring-like patterns of differentiation based on chemical gradients of an acyl-hormoserine lactone (AHL) signal that is synthesized by 'sender' cells is demonstrated. In receiver cells, 'band-detect' gene networks respond to user-defined ranges of AHL concentrations.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 2005
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Programmed population control by cell-cell communication and regulated killing
Article Abstract:
The population-control circuit laid the foundation for using cell-cell communication to programme interactions among bacterial communities. This allows the concept of communication-regulated growth and death to be extended to engineering synthetic ecosystems.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 2004
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