Ligand binding to the porcine adipose tissue beta-adrenergic receptor

Article Abstract:

Labeled radioligands were used to measure beta adrenoreceptor ligand binding in the cell membrane of adipocytes from swines. Binding patterns of tritiated dihydroalprenolol and CGP-12177, and iodine-labeled cyanopindolol were determined and compared to evaluate the influence of the radioligand used on the outcome of the assay. Results showed that the receptors are functional and that binding is correlated with protein concentration. Non-specific binding was also found out to be relatively low for the three radioligands although differences in receptor characterizations were observed depending on the radioligand used.

Evaluation, Ligand binding (Biochemistry), Beta adrenoceptors, Adrenergic beta receptors, Radioligand assay

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Effect of dietary fat and cholesterol level on growing pigs selected for three generations for high or low serum cholesterol at age 56 days

Article Abstract:

A high-fat, high-cholesterol diet did not affect serum cholesterol levels in pigs from high- and low-cholesterol lineages. Hence, there seems to be no interaction between diet and genetic background. Changes in serum total cholesterol concentration may be accompanied by changes in body weight and carcass characteristics. LDL cholesterol appears to be the fraction most affected by genetic selection. Regardless of initial levels, serum cholesterol tended to increase in both groups for the duration of the study.

Swine, Low-cholesterol diet, Low cholesterol diet

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