Phosphorylation and inactivation of the mitotic inhibitor Wee1 by the nim1/cdr1 kinase
Article Abstract:
Biochemical activities of the nim1/cdr1 gene product (Nim1) was observed through induced overproduction of bacterial and baculoviral (bakulaw?) expression systems. Results prove that Nim can be characterized by intrinsic activities associated with serine-kinase, threonine-kinase and tyrosine-kinase. Furthermore, the association of Nim1 and Wee1 was found to lead to the phosphorylation of Wee1. Subsequently, the phosphorylation of Cdc2 on tyrosine is also prevented. These results indicate that Nim1 regulates the mitotic process by rendering Wee1, a mititic inhibitor inactive.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 1993
User Contributions:
Comment about this article or add new information about this topic:
Replication checkpoint requires phosphorylation of the phosphatase Cdc25 by Cds1 or Chk1
Article Abstract:
Checkpoints block mitosis in the cell cycle in response to unreplicated or damaged DNA, usually achieved by preventing activation of the cell-division kinase Cdc2. Chk1 kinase phosphorylates Cdc25, an activator of Cdc2. Mutagenesis has shown that Chk1 functions redundantly with Cds1 kinase at the replication checkpoint. Both kinases are shown to phosphorylate Cdc25 on the same sites.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 1998
User Contributions:
Comment about this article or add new information about this topic:
Nim1 kinase promotes mitosis by inactivating Wee1 tyrosine kinase
Article Abstract:
The function of nim1/cdr 1 gene product (Nim1) in enhancing the mitotic process by inhibting the action of mitosis inhibtor Wee1 tyrosine kinase was studied by studying the impact of direct phosphorylation. Results showed that overproduction of Nim1 led to the hyperphosphorylation of Wee1. Inhibition of the Wee1 kinase also occurred with its phosphorylation by purified Nim1 kinase.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 1993
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Impaired migration but not differentiation of haematopoietic stem cells in the absence of beta1 integrins. PTEN maintains haematopoietic stem cells and acts in lineage choice and leukaemia prevention
- Abstracts: Degeneration in vitro of post-mitotic neurons overexpressing the Alzheimer amyloid protein precursor. Neurotoxicity of beta-amyloid
- Abstracts: Hox gene variation and evolution. DNA answers the call of pipistrelle bat species. Revised phylogeny of whales suggested by mitochondrial ribosomal DNA sequences
- Abstracts: Emerging insights into the genesis of epilepsy. Protease inhibitor implicated. Did radicals strike Lou Gehrig?
- Abstracts: Grb2 mediates the EGF-dependent activation of guanine nucleotide exchange on Ras. Structure of a heparin-linked biologically active dimer of fibroblast growth factor