Single-molecule studies of the effect of template tension on T7 DNA polymerase activity
Article Abstract:
A single-molecule assay based on the differential elasticity of single-stranded and double-stranded DNA has been used to show that mechanical force is produced during the rate-limiting step for DNA replication. It was found that the motor can function against a maximum template tension of around 34 pN. T7 DNA polymerase was found to organize two template bases in the polymerization site during each catalytic cycle. Future single-molecule experiments should establish the mechanism of force-induced exonucleolysis.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 2000
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The bacteriophage phi-29 portal motor can package DNA against a large internal force
Article Abstract:
Optical tweezers have been used to pull on single DNA molecules as they are packaged, showing that the portal complex is a force-producing motor. This motor is extremely strong, functioning against loads of up to 57 pN on average.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 2001
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Structural transitions and elasticity from torque measurements on DNA
Article Abstract:
Tests of the linearity of DNA's twist elasticity, direct measurements of the torsional modulus characterization of torque-induced structural transitions are presented. To understand the structural dynamics of cellular processes such as replication and transcription, knowledge of the elastic properties of DNA is required.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 2003
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