Sizing single DNA molecules
Article Abstract:
Optical contour maximization (OCM) can measure the size of individual DNA molecules more exactly and quickly than existing methods. OCM begins with the staining of DNA molecules by fluorochrome. Electrophoresis then temporarily disperses the DNA through a diffuse agarose matrix. Finally, the molecules are gauged using epifluorescence microscopy with a high degree of linearity between contour length and size. OCM was found to have an accuracy of about 6%, making it preferable to pulsed electrophoresis.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 1992
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Mapping the genome one molecule at a time - optical mapping
Article Abstract:
Optical mapping is a suitable single molecule procedure for developing ordered restriction maps from single DNA molecules. Ordered restriction maps can be developed from yeast artificial chromosomes (YAC) by using fluorescence microscopy. Restriction enzyme cleavage sites are seen as gaps that appear as the DNA fragments relax. The sizing accuracy is similar to routine pulsed-field gel electrophoresis. Optical maps use cosmid, phage YAC and bacterial artificial chromosomes.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 1995
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Genome sequence of enterohaemorrhagic Escherichia coli 0157:H7
Article Abstract:
Research is presented concerning the sequencing of the genome O157:H7 of Escherichia coli to develop techniques for strain detection and to identify genes which cause pathogenesis. A map of the MG1655 and EDL933 genomes is included.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 2001
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