The 1.7 A crystal structure of the regulator of chromosome condensation (RCC1) reveals a seven-bladed propeller
Article Abstract:
The gene which encodes the regulator of chromosome condensation (RCC1) was cloned because it can complement the phenotype of the Hamster cell line tsBN2. This experiences premature chromosome condensation in the G1 phase of the cycle. RCC1 is probably an important part of controlling nucleo-cytoplasmic transport. It is also thought to be able to bind to DNA. X-ray crystallography has been used to study human RCC1, which is shaped like a seven-bladed propeller, and is made up of internal repeats of 51-68 residues on each blade. This explains how a range of mutations occur.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 1998
User Contributions:
Comment about this article or add new information about this topic:
Crystal structure of the GTPase-activating domain of human p120GAP and implications for the interaction with Ras
Article Abstract:
X-ray crystallographic analysis of the catalytically active 334 C-terminal residues, which is the GTPase-activating domain of human p120GAP, gives a molecular structure at 3 angstrom resolution. The GTPase activating proteins (GAP) are involved in the ras mediated regulation. The structure shows that two arginines at 789 and 903 are crucial for the interaction between GAP and ras protein. The Arg 903 interacts with the main chain containing the Arg789. This indicates that the Arg 789 is involved in the catalysis, and it stabilizes the orientation.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 1996
User Contributions:
Comment about this article or add new information about this topic:
Bioorganic synthesis of lipid-modified proteins for the study of signal transduction
Article Abstract:
It is possible to chemically modify Ras proteins with lipopeptides to allow these hybrid proteins to insert into artificial and biological membranes. The neo-lipoproteins have been shown to be efficient tools for biophysical, biochemical and biological experiments. This research used a strategy involving the bacterial expression of a carboxy-terminally truncated non-lipidated protein, the chemical synthesis of differently lipidated peptides representing the C terminus of the proteins, and their covalent coupling.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 2000
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: The crystal structure of the GroES co-chaperonin at 2.8 angstrom resolution. A structural basis of the interactions between leucine-rich repeats and protein ligands
- Abstracts: Social construction, political power, and the allocation of benefits to endangered species. Public opinion on species and endangered species conservation
- Abstracts: The effect of group size on the foraging behaviour of juvenile coho salmon: reduction of predation risk or increased competition?
- Abstracts: A reduced estimate of the number of kilometre-sized near-Earth asteroids. Of asteroids and onions
- Abstracts: Measurement of the spatial coherence of a trapped Bose gas at the phase transition. Direct observation of growth and collapse of a Bose-Einstein condensate with attractive interactions