Trigger factor in complex with the ribosome forms a molecular cradle for nascent proteins
Article Abstract:
A 2.7-Angstrom crystal structure of Escherichia coli trigger factor, the best-characterized chaperone, together with the structure of its ribosome-binding domain in complex with the Haloarcula marismortui large ribosomal subunit is presented. The trigger factor projects the extended domains over the exit of the ribosomal tunnel from its attachment point on the ribosome creating a protected folding space where nascent polypeptides may be shielded from proteases and aggregation.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 2004
User Contributions:
Comment about this article or add new information about this topic:
Placement of protein and RNA structures into a 5 A-resolution map of the 50S ribosomal subunit
Article Abstract:
An electron-density map of the large 50S ribosomal subunit from the bacterium Haloarcular marismortui bacterium has been calculated at 5.0 A resolution. Phases were derived from heavy-atom derivatives, intercrystal density averaging and density-modification methods. Over 300 base pairs of A-form RNA duplex were fitted into the map. A polypeptide exit channel was marked by tungsten cluster compounds bound in a heavy-atom-derivatized crystal.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 1999
User Contributions:
Comment about this article or add new information about this topic:
Crystal structure of the transfer-RNA domain of transfer-messenger RNA in complex with SmpB
Article Abstract:
The 3.2-A-resolution structure of the tRNA-like domain of tmRNA (tmRNA delta) in complex with small protein B (SmpB), a protein essential for biological functions of tmRNA, is reported. The findings indicate that flexible RNA molecule adopts an open L-shaped conformation and SmpB binds to its elbow region, stabilizing the single-stranded D-loop in an extended conformation.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 2003
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: The X factor. US reflects on flying eye for transgenic crops. Speciation reversal
- Abstracts: The promise of emptiness. Complex zeolite structure solved by combining powder diffraction and electron microscopy
- Abstracts: Signal-receiver interplay in the communication of male condition by Asian elephants. Oestrous state dynamics in chemical communication by captive female Asian elephants
- Abstracts: Scratching the surface. Stored in the rings. True blue
- Abstracts: Mullett: Spatchcock of the sea. Silverfish for lucky lips