A PCR detection method for rapid identification of Melissococcus pluton in honeybee larvae

Article Abstract:

European foulbrood is a disease that attacks four- to five-day-old honeybee larvae. The Melissococcus pluton bacterium has been recognized as the cause of this disease. A fast and accurate method for the identification of the bacterium directly from diseased honeybee larvae is described. It uses polymerase chain reaction primers developed from the 16S rRNA gene of the bacterium. The technique takes six hours to complete following overnight incubation of the bee larvae and is particularly valuable given the difficulty in isolating and identifying the bacterium.

Insects, Honeybee, Insect larvae

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Bacterial diversity in Adirondack Mountain lakes as revealed by 16S rRNA gene sequence

Article Abstract:

The bacterial diversity in Adirondack Mountain lakes as revealed by 16S ribosomal RNA gene sequence was investigated. The study used amplification and sequencing of 16S ribosomal DNA. Results show diverse lineages from the class probacteria, the phylum Cytophaga-Flavobacteria-Bacteriodes and the order Actinomycetales. These groups, however, differed among the lakes which suggests that bacterial population structure varies and that aquatic bacterial community structure can be related to water chemistry.

Economic aspects, Ribosomal RNA, Lake ecology, Adirondack Mountain Reserve, New York

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Bacterial florae in larvae of the lake fly Chironomus plumosus

Article Abstract:

Eighteen genera and 29 bacterial species were identified from Chironomus plumosus midge larvae collected from a lake in the early summer periods of 1985 and 1986 in the determination their bacterial flora. Scanning electron microscopy revealed the persistence of the bacteria throughout the intestines. It was found that lake flies are not microbiologically hazardous to the residents of the area.

Flies, Diptera

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