On-line detection of substrate exhaustion by using NAD(P)H fluorescence
Article Abstract:
Aerobic fermentations of the threonine auxotroph Corynebacterium glutamicum were monitored by NAD(P)H fluorescence. Resulting information from the on-line fluorescence signal, such as the rate of change of the fluorescence signal, wasused to evaluate fermentation-related culture metabolic activities, instead of mathematical corrections for inner filter effects. The accuracy of the filteredfluorescence change rate signal for predicting growth-essential substrate depletion was demonstrated.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
User Contributions:
Comment about this article or add new information about this topic:
Cloning of the trp gene cluster from a tryptophan-hyperproducng strain of Corynebacterium glutamicum: identification of a mutation in the trp leader squence
Article Abstract:
Tryptophan biosynthesis gene clusters of the Corynebacterium glutamicum strain were cloned and characterized from Escherichia coli. Resistance of E. coli strains to tryptophan logs was demonstrated. A comparison is made between the amino acid sequence deduced by trpe gene with those encoded from other bacteria. Results indicate the occcurrence of a nonsense mutation which causes constitutive antitermination response.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
User Contributions:
Comment about this article or add new information about this topic:
Production of (R)-3-chloro-1,2-propanediol from prochiral 1,3-dichloro-2-propanol by Corynebacterium sp. strain N-1074
Article Abstract:
The utilization of the bacterial strain Corynebacterium in examining the production of (R)-3-chloro-1,2-propaniedol (MCP) from prochiral 1,3-dichloro-2-propanol (DCP) is discussed. DCP was transformed into MCP by theaddition of glycerol as a carbon source or some chlorinated alcohols to a medium. Results indicate that the formation of enzyme activity can be induced by some metabolites of chlorinated alcohols.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: A PCR detection method for rapid identification of Paenibacillus larvae. Characterization of bacterial and fungal soil communities by automated ribosomal intergenic spacer analysis fingerprints: biological and methodological variability
- Abstracts: The evolution of tandemly repetitive DNA: recombination rules. Active mutator elements suppress the knotted phenotype and increase recombination at the Kn1-0 tandem duplication
- Abstracts: Intraspecific variation in the use of nutrient reserves by breeding female Mallards
- Abstracts: Anabolic incorporation of oxalate by Oxalobacter formigenes. Purification of two nitrate reductases from Xanthomonas maltophila grown in aerobic cultures
- Abstracts: Regulation of cellular differentiation in Caulobacter crescentus