Production of putative virulence factors by Renibacterium salmoninarum grown in cell culture
Article Abstract:
A research on the parthogenicity of Renibacterium salmoninarum utilizing a fish cell culture was conducted to determine the synthesis of intracellular antigens. The virulence of the mechanisms of the R. salmoninarum was also considered. Recombinant DNA technology associated with the poor growth of the organism was the primary methodology used in the study. Findings showed that R. salmoninarum was capable of permeating into and triggering the production of Epithelioma papillosum cyprini cells. (EPC) Furthermore, EPC cells were found to display virulence in the pathogenesis of BKD.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1997
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A controllable gene-expression system for the pathogenic fungus Candida glabrata
Article Abstract:
Incidences of fungal infections have been rising and current therapies depend on polyenes and azoles. The release of the entire nucleotide sequence of Saccharomyces cerevisiae has enabled the identification of all genes in the organism, and a project to sequence the genome of Candida albicans is underway. A system for controlling gene expression in S. cerevisiae has been developed through the use of the tetracycline-responsive element. This method has been applied to C. glabrata, and the effects of repression of TEF3 or TOP2 of C. glabrata on its growth and survival are reported.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1998
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Molecular genetic approaches for the study of virulence in both pathogenic bacteria and fungi
Article Abstract:
The genes that determine the pathogenicity and virulence of pathogens are identified by various molecular genetic selection and screening methods. The screening methods include forming cDNA from the RNA of pathogens, gene fusion, gene transfer, and comparing the genomes of non-pathogenic and pathogenic microorganisms. Directed and random mutagenesis and transposon mutagenesis are also used. Signature-tagged mutagenesis allows the study of different mutants in one host. An alternative to studying some pathogens in animals is to study them in plants.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1996
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