MIG1 overexpression causes flocculation in Saccharomyces cerevisiae
Article Abstract:
MIG1, encoding a C2H2 zinc-finger repressor protein implicated in carbon catabolite repression, was discovered to be involved in non-sexual flocculation of Saccharomyces cerevisiae. Disruption of MIG1 in a flocculent mutant strain of NCYC 227, produced a non-flocculent phenotype. Expression of MIG1 on a 2 micro pRS426 vector in a non-flocculent strain, YM 4134, caused flocculation. MIG1 on a high-copy-number LEU2-d plasmid caused intense flocculation in the same strain. Mutations in the SSN6 and TUP1 genes confer a flocculent phenotype in non-flocculent strains of S. cerevisiae.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1996
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An important role of glutathione and gamma-glutamyltranspeptidase in the supply of growth requirements during nitrogen starvation of the yeast Saccharomyces cerevisiae
Article Abstract:
Enzyme and protein assays were conducted on Saccharomyces cerevisiae to determine the important role of glutathione and gamma-glutamytranspeptidase in the supply of growth requirements during nitrogen starvation of yeasts. Results reveal that the response of glutathione to starvation depends on the nature of the nutrient deprivation. Furthermore, it was found that the central vacuole in the yeast served as an important storage area and provided several transport systems on amino acids and ions using channels or pumps.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1997
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SYR2, a gene necessary for syringomycin growth inhibition of Saccharomyces cerevisiae
Article Abstract:
The growth of Saccharomyces cerevisiae is inhibited by syringomycin, a small cyclic lipodepsipeptide produced by stone-fruit and glass isolates in Pseudomonas syringae. However, some strains of Saccharomyces cerevisiae have developed resistance to syringomycin because of the presence of mutant alleles of the novel yeast gene, SYR2. The role of SYR2 in phospholipid metabolism suggests that phospholipids account for syringomycin's inhibitory action against Saccharomyces cerevisiae.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1996
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- Abstracts: Identification of new genes required for meiotic recombination in Saccharomyces cerevisiae. Meiotic chromosome pairing in triploid and tetraploid Saccharomyces cerevisiae
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