An unusual pectate lyase from a Bacillus sp. with high activity on pectin: cloning and characterization
Article Abstract:
Results demonstrate that pelA gene from Bacillus sp. BP-23 encodes a 23 kDa protein which exhibited homology to bacterial pectate lyases. Data show the enzyme depolymerizes polygalacturonate and pectins with optimal activity at pH 10 and 50 C temperature.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 2000
User Contributions:
Comment about this article or add new information about this topic:
Purification and properties of xylanase A from alkali-tolerant Bacillus sp. strain BP-23
Article Abstract:
The bacillus strain BP-23 produces xylanase A, which has a molecular mass of 32 kDa and an isoelectric point of 9.3. The enzyme's activity is maximum at 50 degree celsius and a pH of 5.5. It is also active at alkaline pHs. N-bromosuccinimide suppresses xylanase activity. Incubation of birchwood bark pulp with xylanase A increases the bleachability of pulp and decreases chlorine dioxide consumption by 38%. The amino-terminal sequence of xylanase A has a conserved sequence of five amino acids.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1995
User Contributions:
Comment about this article or add new information about this topic:
A multidomain xylanase from a Bacillus sp. with a region homologous to thermostabilizing domains of thermophilic enzymes
Article Abstract:
The cloning and sequencing of the gene encoding xylanase C from Bacillus sp BP-23 and the characterization of the enzyme are described. The enzyme XynC discussed is the only xylanase from Bacillus spp that exhibits a multidomain structure with three independent domains. It shows a family IX cellulose-binding domain and a region homologous to thermostabilizing domains. Therefore, Xyn C is unique since it is the only example of an enzyme from the genus Bacillus demonstrating these types of domains.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1999
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Comparative study of pressure-induced germination of Bacillus subtilis spores at low and high pressures. Comparative study of pressure- and nutrient-induced germination of Bacillus subtilis spores
- Abstracts: Structural analysis of Bacillus megaterium KM spore peptidoglycan and its dynamics during germination. Complete spore-cortex hydrolysis during germination of Bacillus subtilis 168 requires SleB and YpeB
- Abstracts: A 22 kb DNA sequence in the cspB-glpPFKD region at 75 degrees on the Bacillus subtilis chromosome. Chaperone-like activities of the CsaA protein of Bacillus subtilis