A natural view of microbial biodiversity within hot spring cyanobacterial mat communities

Article Abstract:

The microbial ecology occurring in the hot spring cyanobacterial mat community found in the effluent channels of the October Spring at the Yellowstone National Park was studied through the 16S rRNA-based cloning, sequencing and oligopeptide probe methods. Results were obtained through the denaturing gradient gel electrophoresis analysis. It was discovered that cyanobacterial populations adapted to narrow temperature-defined niches along the estimated 30 degrees temperature range. Light intensity was the most apparent predictor of changing environmental parameter.

Environmental aspects, Microbial mats, Microbial ecology, Microorganisms, Hot springs, Yellowstone National Park

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Quantification of 16S rRNAs in complex bacterial communities by multiple competitive reverse transcription-PCR in temperature gradient gel electrophoresis fingerprints

Article Abstract:

The major bacterial 16S rRNAs in Drentse A grassland soils in the Netherlands were amplified by reverse transcription-polymerase chain reaction (RT-PCR) with bacterium-specific primers and were differentiated by temperature gradient gel electrophoresis. The sequence-specific efficiency of amplification was measured by competitive PCR and RT-PCR, and identical input quantities of different 16S rRNAs produced identical amplicon yields.

Usage, Soil microbiology, Polymerase chain reaction, Gel electrophoresis, Microbial populations

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• Abstracts: Photosynthate partitioning and fermentation in hot spring microbial mat communities. Cultivation of aerobic chemoorganotrophic proteobacteria and gram-positive bacteria from a hot spring microbial mat

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