Photosynthate partitioning and fermentation in hot spring microbial mat communities

Article Abstract:

The mat cyanobacteria present in the hot springs in Yellowstone National Park produce mainly polysaccharide in the presence of light. The bacteria ferment polysaccharides during the night to produce acetate and propionate. Although the cyanobacteria are photosynthetically active, they show low synthesis of proteins and rRNA that are associated with growth. The mat cores mainly assimilate polyglucose during illumination periods. The rapidly growing Synechococcus sp. strain C1 partitions most of the incorporated 14CO2 into protein, low-molecular-weight metabolites and lipids.

Microbial metabolism, Fermentation

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Denaturing gradient gel electrophoresis used to monitor the enrichment culture of aerobic chemoorganotrophic bacteria from a hot spring cynobacterial mat

Article Abstract:

The denaturing gradient gel electrophoresis separation of 16S rRNA gene fragment helps to study the effect of enrichment culture techniques on the composition of the aerobic chemoorganotrophic bacteria population in Octopus Spring, Yellowstone National Park. Variations in the enrichment conditions, degree of dilution of the inoculum and culture medium composition give 14 unique 16S rRNA sequences. The results reveal the diversity in bacterial populations obtained by enrichment methods in contrast to molecular retrieval and strain purification techniques.

Gel electrophoresis, Microbial populations

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Cultivation of aerobic chemoorganotrophic proteobacteria and gram-positive bacteria from a hot spring microbial mat

Article Abstract:

Cloning and cultivation studies shows that the phenotypically diverse gram-positive bacterial isolates from a cyanobacterial mat from Octopus Spring, Yellowstone National Park, are genetically similar to Bacillus flavothermus. A comparison of cloning and cultivation methods shows that the cultivation methods fail to give the composition of the microbial community. The molecular retrieval technique is unable to describe the less abundant populations in microbial community.

Cloning, Bacteria, Aerobic, Aerobic bacteria

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