A nested reverse transcriptase PCR assay for detection of small round-structured viruses in environmentally contaminated molluscan shellfish
Article Abstract:
A nested reverse transcriptase PCR (RT-PCR) procedure for detecting small round-structured viruses (SRSVs) in molluscan shellfish was developed. An evaluation of the assay in terms of the range of virus strains detected and the sensitivity of detection was conducted. The method was found to detect 15 out of 21 SRSVs, showing that it detects a broad range of SRSVs. It was also found to be more sensitive than the single-round RT-PCR assay by 10 to 1,000 times. Its use for investigating and controlling public health problems caused by consumption of environmentally contaminated shellfish.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
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Detection of small round structured viruses in shellfish by reverse transcription-PCR
Article Abstract:
A sample extraction method involving reverse transcription-PCR is used to identify small round structured viruses (SRSVs) in shellfish. The SRSVs in samples of sewage-contaminated molluscan shellfish and gastroenteritis-outbreak-associated shellfish react with PCR primers on treatment with broadly reactive PCR primers. Sequence analysis of the PCR product reveals that the viruses are SRSVs. Southern blot hybridization support the PCR results.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1995
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Evaluation of potential indicators of viral contamination in shellfish and their applicability to diverse geographical areas
Article Abstract:
Research shows that the presence of human viruses in the shellfish indicates heavy contamination of the geographical areas, which can be identified by analyzing fecal samples for Escherichia coli. Further, the F-specific RNA bacteriophages are related to the presence of viral contamination in shellfish.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2003
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