Actin-related proteins in Anabaena spp. and Escherichia coli
Article Abstract:
The enterobacterium Escherichia coli and the cyanobacteria Anabaena cylindrica and A. variabilis contain prokaryotic actin-related proteins that are biochemically similar to the actin present in eukaryotes. Cellular extracts of the bacteria cross-react with monoclonal antibodies against actin and bind to acetone powder and DNase I extracts. The cells also bind to TRITC-phalloidin that specifically binds to actin. Western blot analysis shows a 60, 43 and 35 kDa band in E. coli cellular extracts, and a 45 and 40 kDa band in A. cylindrica and A. variabilis cellular extracts.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1996
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Pleiotropic effects of potassium deficiency in a heterocystous, nitrogen-fixing cyanobacterium, Anabaena torulosa
Article Abstract:
A study was conducted to analyze the pleiotropic effects of potassium deficiency in the cyanobacterium, Anabaena torulosa. Growth and nitrogenase activity were determined based on chlorophyll a content. Photosynthetic pigment levels were computed from the absorption spectra of dilute suspensions of whole filaments. Results indicated that deprivation led to multiple metabolic impairments during nitrogen-based and diazotrophic growth.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1998
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Actin-related proteins in Actinobacillus pleuropneumoniae and their interactions with actin-binding proteins
Article Abstract:
Research was conducted on the purification and characterization of three prokaryotic actin-related protein isoforms. These proteins, with a molecular weight of 43000 and isoelectric point values of 5.8, 6.15 and 6.2, behaved similarly to eukaryotic actin in their biochemical and immunological properties.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1999
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