Unique and overlapping pollutant stress proteins of Eschericia coli
Article Abstract:
The exposure to of Eschericia coli to nine common environmental pollutants induced the production of 13 to 39 stress proteins. In this study, batch cultures of E. coli K-12 strain were grown in the presence of different pollutants which include benzene, cadmium chloride, 2,4-dichloroaniline, hexachlorobenzene, trichloroethylene, dioctylphtalate, chlorpyrivos and pentacholorphenol. Results showed the sythesis of different stress proteins which were unique to a given chemical. In addition, the induction of chemical stress proteins occurred even in concentrations that did not result in growth inhibition. Thus, it can be used to analyse pollutants even at sublethal level.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
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Styrene metabolism in Exophiala jeanselmei and involvement of a cytochrome P-450-dependent styrene monooxygenase
Article Abstract:
The yeast-like fungus Exophiala jeanselmei metabolizes styrene by oxidizing the vinyl side chain to phenylacetic acid which is then hydroxylated to homogentisic acid. The maximum absorption of the reduced CO-difference spectrum of styrene-grown E. jeanselmei cells is at 450 nm indicating a role for a cytochrome P-450-dependent monooxygenase. The reactions are catalyzed by a styrene monooxygenase, dependent on flavin adenine dinucleotide and NADPH, a phenylacetaldehyde dehydrogenase dependent on NAD+ and a styrene oxide isomerase. Inhibitors of cytochrome P-450 block the monooxygenase activity.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
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Purification and characterization of a trehalose synthase from the basiodiomycete Grifola frondosa
Article Abstract:
A unique trehalose synthase (TSase) that catalyzes the synthesis of trehalose from D-glucose and alpha-D-glucose 1-phosphate was found in the basidiomycete Grifola frondosa. Screening studies showed that basidiomycetes have relatively more trehalose phosphorolysis and synthesis activities than ascomycetes, zygomycetes and mitosporic fungi. TSase activity was found to be common in the fungi investigated and trehalose is synthesized mostly from trehalose-6-phosphate by the dephosphorylating activity of trehalose 6-P-phosphatase.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
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