Advances in development of a genetic system for Thermoanaerobacterium spp.: expression of genes encoding hydrolytic enzymes, development of a second shuttle vector, and integration of genes into the chromosome
Article Abstract:
Advances in the genetic engineering of Thermoanaerobacterium spp. are discussed. This organism can survive at temperatures up to 80 deg. C. and can use xylan, which is a cheap substrate for industrial fermentation.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2000
User Contributions:
Comment about this article or add new information about this topic:
A high-molecular-weight, cell-associated xylanase isolated from exponentially growing Thermoanaerobacterium sp. strain JW/SL-YS485
Article Abstract:
Gel filtration suggests a molecular weight of 350 kDa for cell-associated beta-1,4-xylanase isolated from the cell extract of the exponentially growing Thermoanaerobacterium sp. strain JW/SL-YS485, while gel electrophoresis suggests a molecular weight of 234 kDa for beta-1,4-xylanase. Enzyme assay reveals that beta-1,4-xylanase exhibits highest activity at pH 6.2 and 80 degrees Celsius. Size, location and subunit structure of beta-1,4-xylanase are different from those of other xylanases.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1995
User Contributions:
Comment about this article or add new information about this topic:
Purification and characterization of the alpha-glucuronidase from Thermoanaerobacterium sp. strain JW/SL-YS485, an important enzyme for the utilization of substituted xylans
Article Abstract:
Chromatographic techniques help purify cell-associated alpha-glucuronidase from the Thermoanaerobacterium sp. strain JW/SL-YS485. Analysis of the enzyme reveals the molecular weight as 130,000 and it has a pI of 4.65. Enzyme assay reveals that the enzyme exhibits optimum activity at pH 5.4 and 60 degrees Celsius. 4-methylglucuronosyl xylotetraose is synergistically hydrolyzed by alpha-glucuronidase and beta-xylosidase of the same organism.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1995
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Sequencing of a 65 kb region of the Bacillus subtilis genome containing the lic and cel loci, and creation of a 177 kb contig covering the gnt-sacXY region
- Abstracts: Comparison of PCR and cell culture for detection of enteroviruses in sludge-amended field soils and determination of their transport
- Abstracts: Cultivation and in situ detection of a thermophilic bacterium capable of oxidizing propionate in syntrophic association with hydrogenotrophic methanogens in a thermophilic methanogenic granular sludge
- Abstracts: Dmp1 is haplo-insufficient for tumor suppression and modifies the frequencies of Arf and p53 mutations in Myc-induced lymphomas
- Abstracts: Additive and non-additive genetic architecture of two different-sized populations of Scabiosa canescens. Genetic structure in the nonrewarding, bumblebee-pollinated orchid Calypso bulbosa