Amplified expression of fructose 1,6-biophophatase in Corynebacterium glutamicum increases in vivo flux through the pentose phosphate pathway and lysine production on different carbon sources
Article Abstract:
The deregulated expression of fructose 1,6-biphosphatase (FBPase) in Corynebacterium glutamicum and its impact on growth and lysine production on different carbon sources are described. The overexpression of FBPase might be useful for starch- and molasses-based industrial lysine production with Corynebacterium glutamicum and the redirection of flux toward the pentose phosphate pathway (PPP) can be used in the production of other NADPH-demanding compounds as well as for products directly stemming from the PPP.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
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Comparative metabolic flux analysis of lysine-producing Corynebacterium glutamicum cultured on glucose or fructose
Article Abstract:
Results reveal that optimum levels of lysine production by Corynebacterium glutamicumon fructose involves flux modification through the phosphotransferase system for fructose uptake and amplification of fructose 1,6-bisphosphatase to step-up through the pentose phosphate pathway among others. Metabolic flux analysis indicates that differences in intracellular flux depends on the carbon source of the sugars.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
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Metabolic fluxes in corynebacterium glutamicum during lysine production with sucrose as carbon source
Article Abstract:
Metabolic fluxes in the central metabolism are determined for lysine producing Corynebacterium glutamicum ATCC 21526 with sucrose as a carbon source. The high TCA cycle flux and the significant secretion of dihydroxyacetone and glycerol display interesting targets to be approached by genetic engineers for optimization of the strain examined.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
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