An indirect enzyme immunoassay for the mycotoxin citrinin
Article Abstract:
An indirect enzyme immunoassay (EIA) method of testing the presence of the mycotoxin citrinin is able to detect citrinin in buffer solutions and in wheat flour to which citrinin had been added. The technique involves making a citrinin-glucose oxidase complex which is put on microtiter plates to which rabbit antisera is added. Titration of these microtiter plates yielded good recovery of citrinin. Other mycotoxins such as austdiol, alterariol, ochratoxin A and deoxynivalenol were tested using this method but these toxins do not inhibit the binding of antibodies to the antigens.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1995
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Mycotoxin production by Fusarium species isolated from bananas
Article Abstract:
Some species of Fusarium isolated from bananas and grown on autoclaved corn grain, produce mycotoxins toxic to the larvae of Artemia salina. Eight isolates of Fusarium moniliforme, three of F.proliferatum and all isolates of F.camptoceras prove toxic to the A. salina larvae. Fumonisin B1, fumonisin B2, moniliformin, and zearalenone are some of the mycotoxins determined in the corn culture extracts. Additional compounds generated by the fungal isolates are likely to play an important role in the larval toxicity.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
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Imaging Lactobacillus brevis single cells and microcolonies without a microscope by an ultrasensitive chemiluminescent enzyme immunoassay with a photon-counting television camera
Article Abstract:
A chemiluminescent enzyme immuno assay (CLEIA) is used to visualize single cells and microcolonies of Lactobacillus brevis. This technique provides rapid detection and quantification assays of beer and pitching yeast contaminants. The method for detecting microcolonies is also based on the luminescent assay and is called the microcolony immunoluminescence method. The preparation of the antibody probes and the instrumentation specification of CLEIA are discussed.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
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