An oligopeptide transport gene from Candida albicans
Article Abstract:
The cloning of a novel oligopeptide transport gene (OPT1) from Candida albicans via heterologous expression in Saccharomyces cerevisiae was described. Three observations which indicated the occurrence of this peptide transport system were as follows: mutants resistant to the toxic peptide analogues could transport tetra- and pentapeptides; experiments with radiolabelled compounds showed that tetra- and pentapeptides did not compete with labelled dipeptides and sensitivity to oligopeptides was not regulated by nitrogen source.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1997
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Cloning of a Candida albicans peptide transport gene
Article Abstract:
A mutant strain of Saccharomyces cerevisieae was supplied with a peptide transport system cloned from the genomic material of Candida albicans. The gene was located on the C. albicans DNA by deletion subclones and frameshift mutagenesis to yield a 5.1 kilobase fragment. A protein with 623 amino acids has been deduced from the sequence coding for the transport protein. It can be introduced by plasmid and is similar in structure to a transport gene found in Arabidopsis thaliana.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1995
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The topoisomerase I gene from Candida albicans
Article Abstract:
The cloning and characterization of topoisomerase 1 (TOP1) gene from Candida albicans were described. A conditional TOP1 disruption mutant strain was constructed causing the deletion of one copy of TOP1 which resulted in slight attenuation of virulence. Findings showed that TOP1 gene is not necessary for in vitro growth of Candida albicans but its expression is significant for germ-tube formation, normal cellular structure and virulence in a mouse model system.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1997
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