An unusual genomic position effect on Drosophila white gene expression: pairing dependence, interactions with zeste, and molecular analysis of revertants
Article Abstract:
A P element construct containing the white and rosy genes was inserted in the left arm of the Drosophila chromosome 2, resulting in an unusual position effect characterized by a reduction in white gene expression in the dorsal part of the eye. Reversion analysis showed that enhancing mutations introduced in flanking sequences could act both in cis and in trans, provided that the copies of the white gene are inserted at the same sites in homologous chromosomes. Based on these results, it is proposed that white gene repression is mediated by a repressor molecule, whose bound state is influenced by the pairing of flanking DNA regulatory elements.
Publication Name: Genetics
Subject: Biological sciences
ISSN: 0016-6731
Year: 1992
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Molecular analysis of the zeste-white interaction reveals a promoter- proximal element essential for distant enhancer-promoter communication
Article Abstract:
The zeste-white interaction involves a looping mechanismwhich brings together the enhancer-bound factor and the promoter region. The zetse gene product binds specifically to regions of genes that contain regulatory elements. The nature of the zetse-white interaction was studied by introducing mutations in the zeste binding sites. The results showed that the zeste protein is not absolutely required for distant enhancer action. Further analysis detected a 95-bp promoter-proximal sequence which is required for distant enhancer-promoter interactions. It is proposed that this sequence functions as an anchor for stability of the loop complex.
Publication Name: Genetics
Subject: Biological sciences
ISSN: 0016-6731
Year: 1992
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Temporal patterns of gene expression in the antenna of the adult Drosophila melanogaster
Article Abstract:
Enhancer trap and reporter gene methods were used to examine temporal patterns of gene expression in the adult Drosophila melanogaster. The test focused on the functional relationship between protein presence in the antenna cells and adult age. Some genes expressed patterns that were scaled with life span. A graduated pattern was observed for the adult fly, indicating active gene regulation for the adult life span.
Publication Name: Genetics
Subject: Biological sciences
ISSN: 0016-6731
Year: 1995
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