Assessment of (3H)thymidine incorporation into DNA as a method to determine bacterial productivity in stream bed sediments
Article Abstract:
The applicability of the (3H)thymidine incorporation method in measuring bacterial productivity in stream bed sediments was studied. The results showed that this procedure could yield reasonable values. However, some limitations exist. Firstly, some aerobic and anaerobic bacteria failed to incorporate thymidine into their DNA. Secondly, the determination of isotopic dilution requires a better understanding of the biochemical pathways for thymidine. And lastly, a model for the conversion of DNA incorporation rates into cell production is lacking.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
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Evaluation and simplification of the assimilable organic carbon nutrient bioassay for bacterial growth in drinking water
Article Abstract:
A modified assimilable organic carbon (AOC) bioassay was proposed. All aspects of the AOC bioassay, including inoculum, incubation water, bioassay vessels and other glassware and enumeration technique, were evaluated. Both bioassay organisms, Pseudomonas fluorescens P-17 and Spirillum sp. strain NOX, are available from the American Type Culture Collection and can be easily grown and maintained. Quality control includes an AOC blank and checks for organic carbon limitation and inhibition of the bioassay organisms.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
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Development of a rapid assimilable organic carbon method for water
Article Abstract:
Arapid method for determination of assimilable organic carbon (AOC) was proposed. The time needed to perform the assay is reduced by increasing the incubation temperature, increasing the inoculum density and using the ATP luciferin-luciferase method to quickly enumerate the test organisms. The results show a good correlation between the rapid AOC technique and the plate count procedure.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
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- Abstracts: Role of pore size location in determining bacterial activity during predation by protozoa in soil. Detection of a single genetically modified bacterial cell in soil by using charge coupled device-enhanced microscopy
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