Application of flow cytometry and fluorescent in situ hybridization for assessment of exposures to airborne bacteria
Article Abstract:
Flow cytometry and fluorescent in situ hybridization (FISH) were used to assess exposures to airborne bacteria. The standard culture technique underestimated bioaerosol concentrations by two orders of magnitude when compared to microscopy or flow cytometry. In the FISH technique, aliquots of collection media were incubated, a probe universally complementary to eubacteria, a probe specific for several Pseudomonas species and probe complementary to eubacteria for detection of nonspecific binding. With these probes, FISH permitted quantitative identification of Pseudomonas aeruginosa and Escherichia coli bioaerosols in the exposure chamber without measurable nonspecific binding.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
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A model for the combined effects of temperature and salt concentration on growth rate of food spoilage molds
Article Abstract:
Food spoilage molds Emericella nidulans, Aspergillus niger, Penicillium roqueforti, Trichoderma harzianum and Paecilomyces variotii were used to model mold growth on solid culture medium at different salt concentrations and temperatures. The six-parameter model employed combines the dependence of salt concentration with either the Ratkowsky-type or Rosso-type dependence. The model was proven to be useful in fungal spoilage prediction.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
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