A novel genetically engineered pathway for synthesis of poly(hydroxyalkanoic acids) in Escherichia coli
Article Abstract:
Research describes a novel pathway designed for poly(hydroxyalkanoic acid) (PHA) synthesis from hydroxyfatty acids mediated by three sets of genes each individually coding for butyrate kinase, phosphotransbutyrylase, and PHA synthase enzymes.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2000
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Polyhydroxyalkanoate (PHA) accumulation in sulfate-reducing bacteria and identification of a class III PHA synthase (PhaEC) in Desulfococcus multivorans
Article Abstract:
The chemical compositions of the assumed storage compounds in the three sulfate-reducing bacteria sulfate-reducing bacteria (SRB), Desulfobacterium autotrophicum, Desulfotalea psychrophila and Deculfovibrio vulgaris, were determined. The accumulation of polyhydroxyalkanoates (PHAs) synthase structural genes of Desulfococcus multivornas were identified and the cloned genes were expressed heterologously in other bacteria.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
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Application of the BPEC pathway for large-scale biotechnological production of poly(3-mercaptopropionate) by recombinant Escherichia coli, including a novel in situ isolation method
Article Abstract:
An efficient biotechnological process based on the nonnatural BPEC pathway for production of poly(3-mercaptopropionate) (3MP) homopolymer in a recombinant strain of Escherichia coli at a technical scale was established. A novel in situ process for rapid and convenient isolation of poly(3MP) from the cells in the bioreactor was developed.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
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