Application of the extracellular alpha-amylase gene from Streptococcus bovis 148 to construction of a secretion vector for yogurt starter strains
Article Abstract:
A genetic vector containing the alpha-amylase gene of Streptococcus bovis 148 was used to transform Streptococcus thermophilus ATCC 19528, Lactobacillus delbrueckii subsp. bulgaricus T-11 and Lactococcus lactis subsp. lactis IL1403. All transformed bacteria expressed alpha-amylase and secreted the enzyme. The success of the transformation experiment using the alpha-amylase expression elements has bearing in the transformation of starting strains in yogurt production.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
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Use of glnQ as a counterselectable marker for creation of allelic exchange mutation in group B streptococci
Article Abstract:
A counterselectable marker is reported by using glnQ with gamma-glutamyl hydrazide (GGH) selection and is used to isolate mutant GBS strains that have the chromosomal capsular polysaccharide regulatory gene cpsB replaced by the chloramphenicol acetyltransferase (cat) gene. It was found that efficient allelic exchange mutagenesis in group B streptococci (GBS) was hampered by the lack of a counterselectable marker system.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
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