Assay and properties of acetone carboxylase, a novel enzyme involved in acetone-dependent growth and CO2 fixation in Rhodobacter capsulatus and other photosynthetic and denitrifying bacteria
Article Abstract:
The kinetics of acetone carboxylase activity in Rhodobacter capsulatus was analyzed by utilizing a novel assay for acetone-dependent CO2 fixation in cell-free extracts. Analysis of acetone carboxylase activity in cell-free extracts of Rhodobacter capsulatus based on a novel assay for acetone carboxylation indicated the biotin-independent nature of the novel carboxylase. Furthermore, the acetyl-CoA acted as an activator or component of the acetone carboxylase-mediated reaction mechanism.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1997
User Contributions:
Comment about this article or add new information about this topic:
Acetyl-CoA-dependent pyruvate carboxylase from the photosynthetic bacterium Rhodobacter capsulatus: rapid and efficient purification using dye-ligand affinity chromatography
Article Abstract:
A rapid dye-ligand affinity chromatography method is used in the purification of acetyl-CoA-dependent pyruvate carboxylase (PC) from the photosynthetic bacterium Rhodobacter capsulatus. In this procedure, the PC activity is eluted from the dye column with a low concentration of acetyl-CoA. The enzyme, generated in 75% yield, has a specific activity of 40 U per (mg protein) and a molecular mass of 140000 Da. Acetyl-CoA activates the enzyme and 2-oxoglutarate inhibits it.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1995
User Contributions:
Comment about this article or add new information about this topic:
Evidence for the presence of the reductive pentose phosphate cycle in a filamentous anoxygenic photosynthetic bacterium, Oscillochloris trichoides strain DG-6
Article Abstract:
Research was conducted to demonstrate the operation of the reductive pentose phosphate cycle in Oscillochloris trichoides strain DG-6. Key enzymes of the cycle-rublose-1,5-biphosphate carboxylase/oxygenase and phosphoribulokinase were observed in cell extracts. The addition of cyanide led to complete inhibition of 14CO2 assimilation in O. trichoides DG-6 cels. Results indicate that a functional reductive pentose phosphate cycle is present in O. trichoides DG-6 activity.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1999
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: P13K gamma modulates the cardiac response to chronic pressure overload by distinct Kinase-dependent and independent effects
- Abstracts: A pattern-based method for the identification of microRNA binding sites and their corresponding heteroduplexes
- Abstracts: Impact of the microscale distribution of a Pseudomonas strain introduced into soil on potential contacts in indigenous bacteria
- Abstracts: Cysteine metabolism in Legionella pneumophila: Characterization of an L-cystine-utilizing mutant. Legionella pneumophila in cooling towers : Fluctuations in counts, determination of genetic variability by pulsed-field gel electrophoresis (PFGE), and persistence of PFGE patterns
- Abstracts: Comparative recoveries of Naegleria fowleri amoebae from seeded river water by filtration and centrifugation. Viral contribution of dissolved DNA in the marine environment as determined by differential centrifugation and kingdom probing