Beta-glucuronidase (GUS) transposons for ecological and genetic studies of rhizobia and other Gram-negative bacteria
Article Abstract:
Series of transposons containing gusA gene, encoding beta-glucuronidase show that these transposons are suitable for investigating the population and ecology of bacteria in association with plants and the molecular basis of their interactions. The gusA genes are expressed from regulated and constitutive promoters including tac promoter which is induced by IPTG and nifH promoter. The GUS transposons are formed in a mini-Tn5 systems which can be shifted to Gram-negative bacteria by conjugation. The structure and applications of various transposons are discussed.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1995
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Simultaneous detection of different Rhizobium strains marked with either the Escherichia coli gusA gene or the Pyrococcus furiosus celB gene
Article Abstract:
The combination of the Escherichia coli gusA marker gene and the Pyrococcus furiosus celB gene helps in simultaneously detecting different Rhizobium strains on a plant and the presence of multiple-strains in individual nodules. The gusA gene marked strains and the celB gene marked strains help in the analysis of the whole system of roots, without analyzing the nodules. The celB gene is effective as a marker, since it encodes beta-glucosidase and the beta-galactosidase that are easily inactivated.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
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Diversity of Rhizobia nodulating Phaseolus vulgaris L. in two Kenyan soils with contrasting pHs
Article Abstract:
A comparison of the nodulation in Phaseolus (P.) vulgaris induced by Rhizobium in two different Kenyan soils with pH 4.5 and 6.8 reveals that the bacteria are similar in nitrogen fixing capacity but different in composition of population. The bacteria in the Naivasha soil with a pH 6.8 possess four or more copies of the structural gene for nitrogenase, nifH, and are specific to a host range for nodulation. The Daka-ini soil bacteria has three copies of nifH but are specific only to P. vulgaris.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1995
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