Toluene monooxygenase-catalyzed epoxidation of alkenes

Article Abstract:

Various toluene monooxygenase-producing organisms were tested for ability to oxidize linear alkenes and chloroalkenes 3-8 carbons in length. Each organism, all of them wild-type, degraded all of the alkenes tested. Epoxides were produced when butene, pentene and butadiene were oxidized, but not when hexene or octadiene was. An E. coli strain which expressed the cloned toluene-4-monooxygenase (T4MO) of Pseudomonas mendocina KR1 could oxidize hexene, butene, pentene, and butadiene. It could not oxidize octadiene. A T4MO-deficient variant of P. mendocina KR1 oxidized alkenes that were 5-8 carbons long, but no epoxides were seen. This suggests that multiple alkene-degrading enzymes are in the organism.

United States, Escherichia coli, Microbial enzymes, Cytochemistry, Olefins, Alkenes

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Biodegradation of bis(2-chloroethyl) ether by Xanthobacter sp. strain ENV481

Article Abstract:

The isolation of a new bis(2-chloroethyl) ether (BCEE)-degrading bacterium, Xanthobacter sp. strain ENV481 and the analysis of BCEE degradation products for this strain are described. BCEE degradation by strain ENV478 is facilitated by a monooxygenase-mediated O-dealkylation mechanism and has resulted in the accumulation of 2-chloroacetic acid that is not readily degraded by the strain.

Science & research, All Other Basic Organic Chemical Manufacturing, Industrial organic chemicals, not elsewhere classified, Chloroacetic Acid, Oxidases, Chloroacetic acids, Ethers, Ethers (Class of compounds), Chemical properties

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Chloroform mineralization by toluene-oxidizing bacteria

Article Abstract:

A study on seven toluene-oxidizing bacterial strains shows that Pseudomonas mendocina KR1, Pseudomonas sp. strains ENVPC5 and ENVBF1 are able to mineralize chloroform (CF) to CO2 and chloride ions. The strain ENVBF1 attains the greatest rate of CF oxidation. Escherichia coli DH510B(pRS202), which contains cloned T4MO genes from P. mendocina KR1, also shows the ability to degrade CF. The presence of trichloroethylene inhibits CF oxidation, while acetylene inhibits trichloroethylene oxidation by P. mendocina KR1.

Oxidation, Physiological, Physiological oxidation, Physiological regulation, Chloroform, Biomineralization

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