Cell cycle-dependent polar localization of chromosome partitioning proteins in Caulobacter crescentus
Article Abstract:
The role of parA and parB genes in the process of chromosomal partitioning was analyzed by cloning the homologs of the plasmid partitioning genes from the development bacterium Caulobacter crescentus. Analysis of chromosomal DNA partitioning in Caulobacter crescentus indicated the specific binding of ParB to DNA sequences located downstream of the parB operon. A periodic bipolar localization pattern in late predivisional cells was also detected after the completion of DNA replication indicating the role of ParA and ParB as components of a mitotic-like apparatus in bacterial cells.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1997
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Separation of sister chromatids in mitosis requires the Drosophila pimples product, a protein degraded after the metaphase/anaphase transition
Article Abstract:
A gene that regulates the mechanical segregation of chromatids during the metaphase and anaphase stages of mitosis in Drosophila was identified. The pimples gene's role in mitosis was determined when mutations of this gene caused sister chromatids to fail to draw apart during metaphase, so that the next generation of mitosis shows a doubling of chromatid arms attached to the centromeres. The gene was found to code for the production of a protein whose degradation product during mitosis may serve as a signal for sister chromatids segregated to opposite poles of the splitting cell.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1996
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Kinetochore localization of murine Bub1 is required for normal mitotic timing and checkpoint response to spindle damage
Article Abstract:
The biochemical functions of the murine homolog of BUB1 in cellular apoptosis were analyzed by utilizing monoclonal antibodies against the amino acids 332-337 of the protein. Immunoblotting analysis of the murine homolog of BUB1 indicated its ability to localize to the kinetochore. However, the murine Bub1 did not affect cell cycle arrest follwing spindle damge in mammalian cells. Furthermore, similar cell lines exhibited different biochemical responses after mitosis in the absence of the spindle.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1997
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