Characterization of Listeria monocytogenes isolates by esterase electrophoresis

Article Abstract:

Alpha-naphthyl-propionyl esterase (alpha-NPE) electrophoresis is a quick and efficient method for the typing of Listeria monocytogenes strains in raw-milk, nondairy, clinical, and veterinary samples. The results agree well with those obtained from the multilocus enzyme electrophoresis (MEE) method. The MEE method gives information regarding the mobility of the NPEs and other enzymes, and is more specific. Using the alpha-NPE method, 17 different alpha-NPE types have been assigned to the 219 L. monocytogenes isolates obtained from various sources.

Identification and classification, Electrophoresis

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Characterization of recurrent and sporadic Listeria monocytogenes isolates from raw milk and nondairy foods by pulsed-field gel electrophoresis, monocin typing, plasmid profiling, and cadmium and antibiotic resistance determination

Article Abstract:

Pulsed-field gel electrophoresis can provide results similar to multilocus enzyme electrophoresis analysis in the study of recurrent and sporadic Listeria monocytogenes isolates. Characteristics that allow the organism to persist in food include the production of type E monocin, plasmid carriage, and resistance to cadmium.

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Molecular typing by pulsed-field gel electrophoresis of Spanish animal and human Listeria monocytogenes isolates

Article Abstract:

Contaminated silage, potatoes, and maize stalks appear to be linked to many cases of animal listeriosis in Spain. Pulsed-field gel electrophoresis identified 67 different pulsotypes in a study of sheep, cattle, humans, and feedstuffs. Many strains taken from clinical outbreaks had identical pulsotypes.

Spain, Contamination, Silage

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