Characterization of a second cell-associated Arg-specific cysteine proteinase of Porphyromonas gingivalis and identification of an adhesin-binding motif involved in association of the prtR and prtK proteinases and adhesins into large complexes
Article Abstract:
A study was conducted to characterize the second Arg-specific proteinase of Porphyromonas gingivalis and the cloning and sequence analysis of its gene, prtRII. P. gingivalis W50 was prepared in batch culture and harvested at late-exponential phase by centrifugation. Polymerase chain reaction was utilized to amplify a 991 bp fragment supporting the internal Eco721 site encoded by prtRII. Results indicated that prtR resulted from the gene duplication and recombination of ancestral prtRII and adhesin genes.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1998
User Contributions:
Comment about this article or add new information about this topic:
Secretion, interaction and assembly of two O-glycosylated cell wall antigens from Candida albicans
Article Abstract:
A monoclonal antibody (3A10) was raised against the material released from the Candida albicans mycelial cell wall by zymolyase digestion. It appears that after secretion, parts of the O-glycosylated antigen molecules are transferred to an N-glycosylated protein or proteins. The process that results in the final molecular product of each antigen and distribution in the cell walls is discussed.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 2001
User Contributions:
Comment about this article or add new information about this topic:
Different adhesins for type IV collagen on Candida albicans: identification of a lectin-like adhesin recognizing the 7S(IV) domain
Article Abstract:
Interaction of Candida albicans yeast cells with the three main domains of type IV collagen, a major basement membrane glycoprotein, have been analyzed. The pathogen adhered to the three immobilized domains by different mechanisms. A lectin-like adhesin that recognizes the 7S(IV) domain has been identified.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 2001
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Impaired secretion of a hydrophobic cutinase by Saccharomyces cerevisiae correlates with an increased association with immunoglobulin heavy-chain binding protein (BiP)
- Abstracts: Coupling estimated effects of QTLs for physiological traits to a crop growth model: predicting yield variation among recombinant inbred lines in barley
- Abstracts: The partitioning activity of the RK2 central control region requires only incC, korB and KorB-binding site OB3 but other KorB-binding sites form destabilizing complexes in the absence of OB3
- Abstracts: Effects of specific inhibitors on anammox and denitrification in marine sediments. Measurement of denitrification in sediments with the (super)15 N isotope pairing technique
- Abstracts: Source bioaerosal concentration and rRNA gene-based identification of microorganisms aerosolized at a flood irrigation wastewater reuse site