The partitioning activity of the RK2 central control region requires only incC, korB and KorB-binding site OB3 but other KorB-binding sites form destabilizing complexes in the absence of OB3
Article Abstract:
The central control region (ctl) of the broad-host-range IncP plasmid RK2 was studied to determine its capability in stabilizing heterologous replicons and the genes required for this activity. The ctl contains three operons: korAB operon, krfA monocistronic operon and upf54.8-up54.4 bicistronic operon. The korAB operon encoded the korA, incC, korB, korF and korG. It was discovered that incC and korB were required for phenotypic stability. The inactivation of the KorB binding site OB3 produced a region which destabilized the unstable plasmid.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1998
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Identification of a 60 kb region of the chromosome of Pseudomonas fluorescens NCIB 10586 required for the biosynthesis of pseudomonic acid (mupirocin)
Article Abstract:
Mupirocin is a polyketide antibiotic that prevents the production of isoleucyl-tRNA and is made by the Pseudomonas fluorescens NCIB 10586 bacterium. Analysis with mutants and insertions of the bacterium pinpointed the region, which was determined to be 60 kb in size, that likely makes the mupirocin. The mutants used to determine the region were the 332 Tn5 and Tn1725 types. The genes that code for the mupirocin have not yet been identified.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1995
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Quorum-sensing-dependent regulation of biosynthesis of the polyketide antibiotic mupirocin in Pseudomonas fluorescens NCIMB 10586
Article Abstract:
Research demonstrates that the mup cluster of genes that are involved in mupirocin biosynthesis in Pseudomonas fluorescens are controlled by a quorum-sensing-dependent regulatory system. Data indicate that the mup cluster genes, mupR and mupI, are involved in the mupirocin biosynthetic pathway and also activate the mup operon transcription.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 2001
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