Characterization of the prolyl dipeptidyl peptidase gene (dppIV) from the Koji mold Aspergillus oryzae
Article Abstract:
A study was conducted to clone the Aspergillus oryzae gene encoding a prolyl dipeptidyl peptidase (DPPIV) and to examine the peptide profiles of a wheat gluten-containing medium hydrolyzed by wild-type or dppIV disruptant strains. The RNease total RNA purification kit was utilized to determine total RNA from A. oryzae TK3 mycelium. Results indicated that the disruption of the dppIV gene eliminated all detectable prolyl dipeptidyl peptidase activities.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
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Isolation and characterization of acidocin A and cloning of the bacteriocin gene from Lactobacillus acidophilus
Article Abstract:
High-performance liquid chromatography suggests a molecular weight of 6,500 Da for acidocin A, a bacteriocin isolated from Lactobacillus acidophilus TK9201. Oligonucleotide probes based on the sequence of first 16 amino acids on the N terminus of the acidocin A help identify the acidocin A structural gene acdA. An immunity function of acidocin A is encoded by an additional open reading frame ORF2 in acdA.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1995
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Influence of fermentation medium composition on physicochemical surface properties of Lactobacillus acidophilus
Article Abstract:
The influence of the simple and complex basic components of a fermentation medium on the surface properties of Lactobacillus acidophilus NCC2628 is analyzed using electrophoresis, interfacial adhesion and X-ray photonelectron spectroscopy and by transmission electron microscopy. The results show that expression of the S-layer protein is dependent on the protein source of the fermentation medium.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
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