Characterization of transcription initiation and termination signals of the proteinase genes of Lactococcus lactis Wg2 and enhancement of proteolysis in L. lactis
Article Abstract:
Proteolytic activity in Lactococcus lactis Wg2 requires the expression of prtP, which encodes a serine proteinase, and prtM, which is involved in the processing of the proteinase. The transcriptional start and termination signals for these genes were determined. The localization of these sequences allowed for further characterization. The results showed that the Wg2 promoter is not as strong as previously isolated promoters. The proteolytic activity of the bacterium was increased by orienting the two prt genes in tandem, and replacing the native promoter with a stronger one.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
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Molecular analyses of the lactococcin A gene cluster from Lactococcus lactissubsp. lactis biovar diacetylactis WM4
Article Abstract:
The genes responsible for bacteriocin production and immunity in Lactococcus lactis subsp. lactis biovar diacetylactis WM4 were isolated and characterized. Sequence analysis showed that a gene cluster composed of four open reading frames (ORFs) is responsible for bacteriocin production and immunity. The last two ORFs are identical to the lactococcin A production and immunity genes. Analysis of the deduced proteins of the other two ORFs indicates that they are involved in lactococcin A secretion and processing.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
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Molecular characterization of the integration of the lactose plasmid from Lactococcus lactis subsp. cremoris SK11 into the chromosome of L. lactis subsp. lactis
Article Abstract:
XbaI-XhoI fragment of a Lac plasmid pSK11L was subcloned for used as probe in Southern hybridization analyses of the natural lactococcal integration of pSK11L into the chromosome of Lactococcus lactis subspecies lactis LMO230. The two junction fragments followed a Campbell-like, single-cross over mechanism. In addition, the presence of the 1.6-kb fragment in a recombinant vector allowed the Rec-dependent, single-crossover integration of the plasmid into the lactoccal chromosome.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
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